Mitochondrial protein turnover

Method:

Using protein degradation and abundance data aggregated from previous studies with ~60% proteome coverage, calculate protein synthesis/degradation rate (at proteostasis) as:

Rate = abundance * degradation rate constant per copy

Total rate = sum of individual rates

Used the error propagation formula (http://en.wikipedia.org/wiki/Propagation_of_uncertainty) to calculate uncertainty if protein abundances and degradation rates are uncorrelated (this is an assumption).

Then used Caladis to account for incomplete coverage and uncertainty in mitochondrial quantity per cell: www.caladis.org/compute/?q=%24turnoverRate%2F(%24proteomeCoverage*%24numMitosPerCell)&v=turnoverRate%3Anorm%2C1987%2C141%3BproteomeCoverage%3Aunif%2C0.5%2C0.7%3BnumMitosPerCell%3Aunif%2C10%2C50&x=off&n=m&h=fd&a=rad&_=1430315528285